Nikon ECLIPSE LV150 optical microscope

The optical microscope in the cleanroom is a Nikon ECLIPSE LV150 optical microscope.

The manufacturers manual can be found here: nikon_lv150-150a_manual.pdf

DO's and DONT's

Saving an image with annotations

To save annotations/measurements on an image, first capture the image. Make sure the correct objective is selected at which you took the picture, otherwise the measurements will be off.
Use one of the Annotations and measurement tools to make your measurements. Use the option 'Burn overlay', shortcut key 'Y', to burn the annotation layer into your captured image. Now you can save the image.

Stitching images together, creating a sample overview

With the option 'Scan large image' you can create an overview picture of your complete sample. The microscope starts at the upper left corner of the sample takes a picture, moves the stage automatically and continues to scan the complete sample. Make sure you deselect 'image registration', otherwise the software will likely crash.
To get extremely high resolution images you can do this at high magnifications.